Building a Better Gut Microbiota
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Prebiotic, probiotic and synbiotic strategies for success
“Happiness for me is largely a matter of digestion.” ― Lin Yutang, The Importance of Living
As Chinese scholar Lin Yutang observed, a healthy gut is a core component of a happy life. Each person’s gut contains approximately 38 trillion microbes, comprising hundreds of different species (collectively, the microbiota).,, And we now know that a healthy gut largely depends upon having healthy gut microbiota., When everything is going smoothly, beneficial species dominate over the harmful ones, protecting us from indigestion, infections, inflammation, and other troubles.
A healthy microbiota supports not only the gut, but also every major organ in the body including the heart, brain, liver,, kidneys, bones, and even the skin, resulting in a “glow of health.” Conversely, imbalances in the microbiota are associated with allergies, Alzheimer’s disease, asthma, autism and learning challenges, cardiovascular disease, diabetes, eczema, indigestion, metabolic syndrome, and many other ailments.,
It stands to reason that if we can help the beneficial bacteria grow and thrive, we may be able to improve our overall health. The question then becomes: can we really build a better microbiota?
How to “seed” and “feed” the microbiota
A healthy diet, balanced lifestyle, and targeted supplementation can help support the microbiota. We can “seed” the gut with beneficial bacteria by consuming fermented foods or probiotic supplements, and we can “feed” the beneficial bacteria by ingesting foods or supplements containing prebiotics and other nutrients that help them grow.
We can “seed” the gut with beneficial bacteria by consuming fermented foods or probiotic supplements, and we can “feed” the beneficial bacteria by ingesting foods or supplements containing prebiotics and other nutrients that help them grow.
Probiotics are live microorganisms that have been demonstrated to exert positive effects on health when administered in adequate amounts.,,, They also may modify the balance of the intestinal flora, reducing levels of the microorganisms associated with dysbiosis, and restoring levels of those associated with a healthy microbiome., They can be obtained by eating fermented foods such as yogurt and kefir, and/or by probiotic supplementation.
You can often (but not always) find out which bacterial species are in a particular yogurt or kefir brand by reading the label. The most commonly used probiotics are species of Lactobacillus (for example, Lactobacillus acidophilus or L. acidophilus) and Bifidobacterium (for example, Bifidobacterium lactis or B. lactis.) Bifidobacterium is especially important because its abundance declines with age and antibiotic use., Many fermented dairy products are not fortified with Bifidobacterium, or have low concentrations of live probiotics overall, making supplementation a more reliable choice in many cases.
A great way to support a healthy microbiota, gut, and body is to consume plenty of dietary fiber. Bananas, oats, artichokes, and certain other vegetables and whole grains are especially good for the gut because of their high prebiotic content. Prebiotics are water-soluble fibers that boost the growth of beneficial bacteria. They are not digested by human enzymes, so they pass through the small intestine into the colon, where beneficial bacteria use them as fuel for growth. In addition to prebiotics, certain other dietary nutrients including polyphenols and omega-3 fatty acids help stimulate the growth of “good” bacteria.
You may have heard of inulin, fructooligosaccharides (FOS), or galactooligosaccharides (GOS), all of which are prebiotics. Only non-digestible oligosaccharides that are “bifidogenic” (increase bifidobacteria) meet the criteria for prebiotics., However, some prebiotics are more bifidogenic than others. Most Bifidobacterium species can grow on FOS and GOS,, but not on inulin.,
Xylooligosaccharides: prebiotics found in bamboo
The class of prebiotics known as xylooligosaccharides, (pronounced zy-low-all-ih-go-saccharides, or XOS) occur naturally in bamboo shoots, fruits, vegetables, milk, and honey. Although XOS can be extracted from bamboo, they are more economically produced by enzymatic treatment of corn cob residues.,
The 5-carbon (pentose) structure of XOS is fundamentally different from other prebiotics; this makes XOS highly selective for bifidobacteria, which have the enzymes necessary to break down pentose sugars.,,,, Several Lactobacillus species are capable of fermenting XOS to a lesser extent.,, Importantly, XOS cannot be utilized by pathogenic bacteria.
XOS was shown to boost Bifidobacterium numbers in vitro (in the lab) and in vivo (in the body), and to enhance the production of butyrate,, a short-chain fatty acid associated with gastrointestinal health. Butyrate is a result of cross-feeding: it is made by other beneficial bacteria that feed on the products of bifidobacteria fermentation. Like a domino effect, the fermentation of XOS has other benefits: it increases mucus production by gastrointestinal (goblet) cells. A healthy mucus layer helps “good” bacteria attach to the colon, while it protects against gut pathogens.,,
The daily ingestion of XOS by human subjects induced significant increases in fecal Bifidobacterium counts within four to eight weeks.,,,,, XOS supplementation also decreased the abundance of Clostridium perfringens, an opportunistic pathogen., The health benefits of XOS typically have been observed at one to five grams per day, a lower dose than required for prebiotics such as FOS.,,,,,
Synbiotics with Bifidobacterium and XOS
When British scientist Glenn Gibson introduced the concept of prebiotics in 1995, he speculated as to the additional benefits if prebiotics were combined with probiotics to form what he termed synbiotics. The term “synbiotic” refers to “a combination of synergistically acting probiotics and prebiotics, where a selected component introduced to the gastrointestinal tract should selectively stimulate growth and/or activate the metabolism of a physiologic intestinal microbiota, thus having a beneficial effect on the host’s health.”
The term “synbiotic” refers to a combination of synergistically acting probiotics and prebiotics.
Note that the word “synbiotic” is not the same as “symbiotic”, which refers to an interaction between two different organisms living in close physical association.
Because the word “synbiotic” implies synergism, this term is reserved for products in which the prebiotic compound selectively favors the probiotic organism. The combination of B. lactis with XOS fits this description. In a simulated colon model, the addition of XOS not only increased overall numbers of bifidobacteria, but produced a tenfold increase in the abundance of B. lactis. The results indicate that XOS plus B. lactis would form a successful synbiotic combination.
Large controlled trials are needed to assess the clinical effects in humans, but a preliminary study suggested the synbiotic XOS plus B. lactis improved the self-reported quality of life in otherwise healthy individuals, compared to the probiotic alone. XOS is well tolerated and has a long history of safe use. Because a lower dose is needed than FOS for similar beneficial effects, XOS also is better tolerated, with less gas and bloating than is commonly seen with FOS.
A healthy gut is essential for a healthy and happy life, and a healthy gut requires a well-balanced microbiota! To support your microbiota, focus on a high-fiber diet; include fermented food products such as kefir; and consider probiotic, prebiotic, and/or synbiotic supplementation to “seed” and “feed” beneficial bacteria. Prebiotics and synbiotics containing XOS are highly selective for beneficial bifidobacteria, are well-tolerated, and support an improved quality of life.
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 Yutang L. The Importance of Living. New York; William Morris and Company, Inc.: First Quill Edition; September 1998.
 Lloyd-Price J, et al. The healthy human microbiome. Genome Med. 2016 Apr 27;8(1):51.
 Valdes AM, et al. Role of the gut microbiota in nutrition and health. BMJ. 2018 Jun 13;361:k2179.
 Sender R, et al. Revised estimates for the number of human and bacteria cells in the body. PLoS Biol. 2016 Aug 19;14(8):e1002533.
 Mitsuoka T. Intestinal flora and human health. Asia Pac J Clin Nutr. 1996 Mar;5(1):2-9.
 Dimidi E, et al. Mechanisms of action of probiotics and the gastrointestinal microbiota on gut motility and constipation. Adv Nutr. 2017 May 15;8(3):484-94.
 Tang WH, et al. Gut microbiota in cardiovascular health and disease. Circ Res. 2017 Mar 31;120(7):1183-96.
 Spielman LJ, et al. Unhealthy gut, unhealthy brain: the role of the intestinal microbiota in neurodegenerative diseases. Neurochem Int. 2018 Aug 14;120:149-63.
 Han R, et al. Mechanistic and therapeutic advances in non-alcoholic fatty liver disease by targeting the gut microbiota. Front Med. 2018 Sep 4.
 Altamirano-Barrera A, et al. The role of the gut microbiota in the pathology and prevention of liver disease. J Nutr Biochem. 2018 Mar 16;60:1-8.
 Xu KY, et al. Impaired renal function and dysbiosis of gut microbiota contribute to increased trimethylamine-N-oxide in chronic kidney disease patients. Sci Rep. 2017 May 3;7(1):1445.
 McCabe L, et al. Prebiotic and probiotic regulation of bone health: role of the intestine and its microbiome. Curr Osteoporos Rep. 2015 Dec;13(6):363-71.
 Erdman SE, Poutahidis T. Probiotic ‘glow of health’: it’s more than skin deep. Benef Microbes. 2014 Jun 1;5(2):109-19.
 Tojo R, et al. Intestinal microbiota in health and disease: role of bifidobacteria in gut homeostasis. World J Gastroenterol. 2014 Nov 7;20(41):15163-76.
 Gagliardi A, et al. Rebuilding the gut microbiota ecosystem. Int J Environ Res Public Health. 2018 Aug 7;15(8).
 Sirisinha S. The potential impact of gut microbiota on your health: current status and future challenges. Asian Pac J Allergy Immunol. 2016 Dec;34(4):249-64.
 Ejtahed HS, et al. Effect of probiotic yogurt containing Lactobacillus acidophilus and Bifidobacterium lactis on lipid profile in individuals with type 2 diabetes mellitus. J Dairy Sci. 2011 Jul;94(7):3288-94.
 Ringel-Kulka T, et al. Probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium lactis Bi-07 versus placebo for the symptoms of bloating in patients with functional bowel disorders: a double-blind study. J Clin Gastroenterol. 2011 Jul;45(6):518-25.
 Eskesen D, et al. Effect of the probiotic strain Bifidobacterium animalis subsp. lactis, BB-12®, on defecation frequency in healthy subjects with low defecation frequency and abdominal discomfort: a randomized, double-blind, placebo-controlled, parallel-group trial. Br J Nutr. 2015 Nov 28;114(10):1638-46.
 Hill C, et al. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014 Aug;11(8):506-14.
 Forssten S, et al. Influence of a probiotic mixture on antibiotic induced microbiota disturbances. World J Gastroenterol. 2014 Sep 7;20(33):11878-85.
 Gargari G, et al. Consumption of a Bifidobacterium bifidum strain for 4 weeks modulates dominant intestinal bacterial taxa and fecal butyrate in healthy adults. Appl Environ Microbiol. 2016 Sep 16;82(19):5850-9.
 Arboleya S, et al. Gut bifidobacteria populations in human health and aging. Front Microbiol. 2016 Aug 19;7:1204.
 Rivière A, et al. Bifidobacteria and butyrate-producing colon bacteria: importance and strategies for their stimulation in the human gut. Front Microbiol. 2016 Jun 28;7:979.
 Yang Y, et al. Association between dietary fiber and lower risk of all-cause mortality: a meta-analysis of cohort studies. Am J Epidemiol. 2015 Jan 15;181(2):83-91.
 Slavin J. Fiber and prebiotics: mechanisms and health benefits. Nutrients. 2013 Apr 22;5(4):1417-35.
 Ercolini D, Fogliano V. Food design to feed the human gut microbiota. J Agric Food Chem. 2018 Apr 18;66(15):3754-8.
 Dueñas M, et al. A survey of modulation of gut microbiota by dietary polyphenols. Biomed Res Int. 2015;2015:850902.
 Costantini L, et al. Impact of omega-3 fatty acids on the gut microbiota. Int J Mol Sci. 2017 Dec 7;18(12).
 Pokusaeva K, et al. Carbohydrate metabolism in bifidobacteria. Genes Nutr. 2011 Aug;6(3):285-306.
 Pandey KR, et al. Probiotics, prebiotics and synbiotics- a review. J Food Sci Technol. 2015 Dec;52(12):7577-87.
 Rossi M, et al. Fermentation of fructooligosaccharides and inulin by bifidobacteria: a comparative study of pure and fecal cultures. Appl Environ Microbiol. 2005 Oct;71(10):6150-8.
 Davis LM, et al. Barcoded pyrosequencing reveals that consumption of galactooligosaccharides results in a highly specific bifidogenic response in humans. PLoS One. 2011;6(9):e25200.
 Scott KP, et al. Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro. FEMS Microbiol Ecol. 2014 Jan;87(1):30-40.
 Saville B, Saville S. Xylooligosaccharides and arabinoxylanoligosaccharides and their application as prebiotics. Applied Food Biotechnology. 2018 Jul 2;5(3):121-30.
 Jain I, et al. Xylooligosaccharides: an economical prebiotic from agroresidues and their health benefits. Indian J Exp Biol. 2015 Mar;53(3):131-42.
 Vázquez MJ, et al. Xylooligosaccharides: manufacture and applications. Trends in Food Science & Technology. 2000 Nov 1;11(11):387-93.
 Xiao X, et al. Autohydrolysis of bamboo (Dendrocalamus giganteus Munro) culm for the production of xylo-oligosaccharides. Bioresource Technology. 2013 Jun 1;138:63-70.
 Crittenden R, et al. In vitro fermentation of cereal dietary fiber carbohydrates by probiotic and intestinal bacteria. J Sci Food Agric. 2002 Jun;82(8):781-9.
 Gilad O, et al. Combined transcriptome and proteome analysis of Bifidobacterium animalis subsp. lactis BB-12 grown on xylo-oligosaccharides and a model of their utilization. Appl Environ Microbiol. 2010 Nov;76(21):7285-91.
 Carlson JL, et al. Prebiotic dietary fiber and gut health: comparing the in vitro fermentations of beta-glucan, inulin and xylooligosaccharide. Nutrients. 2017 Dec 15;9(12).
 Mäkeläinen H, et al. Xylo-oligosaccharides and lactitol promote the growth of Bifidobacterium lactis and Lactobacillus species in pure cultures. Benef Microbes. 2010 Jun;1(2):139-48.
 Mäkeläinen H, et al. Xylo-oligosaccharides enhance the growth of bifidobacteria and Bifidobacterium lactis in a simulated colon model. Benef Microbes. 2010 Mar;1(1):81-91.
 Li Z, et al. In vitro study of the prebiotic xylooligosaccharide (XOS) on the growth of Bifidobacterium spp and Lactobacillus spp. Int J Food Sci Nutr. 2015;66(8):919-22.
 Van Laere KM, et al. Fermentation of plant cell wall derived polysaccharides and their corresponding oligosaccharides by intestinal bacteria. J Agric Food Chem. 2000 May;48(5):1644-52.
 Lecerf JM, et al. Xylo-oligosaccharide (XOS) in combination with inulin modulates both the intestinal environment and immune status in healthy subjects, while XOS alone only shows prebiotic properties. Br J Nutr. 2012 Nov 28;108(10):1847-58.
 Pham VT, et al. The effects of fermentation products of prebiotic fibers on gut barrier and immune functions in vitro. PeerJ. 2018 Aug 10;6:e5288.
 Liévin-Le Moal V, Servin AL. The front line of enteric host defense against unwelcome intrusion of harmful microorganisms: mucins, antimicrobial peptides, and microbiota. Clin Microbiol Rev. 2006 Apr;19(2):315-37.
 Gill N, et al. Roadblocks in the gut: barriers to enteric infection. Cell Microbiol. 2011 May;13(5):660-9.
 Ebersbach T, et al. Xylo-oligosaccharides inhibit pathogen adhesion to enterocytes in vitro. Res Microbiol. 2012 Jan;163(1):22-7.
 Lin SH, et al. Prebiotic effects of xylooligosaccharides on the improvement of microbiota balance in human subjects. Gastroenterology Res Pract. 2016;2016: 5789232.
 Chung YC, et al. Dietary intake of xylooligosaccharides improves the intestinal microbiota, fecal moisture, and pH value in the elderly. Nutrition Research. 2007 Dec 1;27(12):756-61.
 Finegold SM, et al. Xylooligosaccharide increases bifidobacteria but not lactobacilli in human gut microbiota. Food Funct. 2014 Mar;5(3):436-45.
 Na MH, et al. Effects of xylooligosaccharide intake on fecal bifidobacteria, lactic acid and lipid metabolism in Korean young women. Korean J. Nutr. 2007;40:154-61.
 Childs CE, et al. Xylo-oligosaccharides alone or in synbiotic combination with Bifidobacterium animalis subsp. lactis induce bifidogenesis and modulate markers of immune function in healthy adults: a double-blind, placebo-controlled, randomized, factorial cross-over study. Br J Nutr. 2014 Jun 14;111(11):1945-56.
 Yang J, et al. Xylooligosaccharide supplementation alters gut bacteria in both healthy and prediabetic adults: a pilot study. Front Physiol. 2015 Aug 7;6:216.
 Sheu WH, et al. Effects of xylooligosaccharides in type 2 diabetes mellitus. J Nutr Sci Vitaminol. 2008;54(5):396-401.
 Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr. 1995 Jun;125(6):1401-12.
 Rodiño-Janeiro BK, et al. A review of microbiota and irritable bowel syndrome: future in therapies. Adv Ther. 2018 Mar;35(3):289-310.
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Marina MacDonald, MS, PhD
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